ZymoPURE Plasmid Miniprep Kit, 50 Preps, Zymo Research, Mfr#: ZD4209

Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 μg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 μg/μl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications. As an added convenience, the ZymoPURE Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Highest Yield: Purify up to 100 μg of plasmid DNA in as little as 25 μl directly from a spin-column. Transfection-Grade: 50,000 times fewer endotoxins than industry leading minipreps.BAC/YAC/PAC Ready: Purify DNA up to ~200 kb.
Storage Temperature: Room Temperature
Storage Conditions: Room Temperature
Applicable For: Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications. Binding Capacity: 100 μgCulture Input: 5 mLElution Volume: 25 μl Endotoxin Levels: 1 EU/μg Equipment: Microcentrifuge and/or vacuum manifoldFormat: Spin Column Processing Time: 15 min Purity: Typical Abs 260/280 1.8 and Abs 260/230 2.0 Recommended Sample Volume: 5.0 ml Size Range: Up to 25 kb Yield: Up to 100 μg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized.Shipping Weight: 1 lbs
Shipping Dimensions: 7.00 x 5.00 x 4.50
Research or further manufacturing use only, not for food or drug use.